Goat anti-TIM50 Antibody |
| Principle Name | TIM50 |
| Official Symbol | TIMM50 |
| All Name and Symbols | TIMM50; TIM50L; translocase of inner mitochondrial membrane 50 homolog (yeast); Tim50-like protein; homolog of yeast Tim50; translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae); MGC102733; TIM50 |
| Accession Number(s) | NP_001001563.1 |
| Peptide Sequence | CDVRTVLEHYALEDD, is from internal region |
| Human Gene ID(s) | 92609 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Experiments gave bands at approx 40kDa in Human Heart lysate after 0.5µg/ml antibody staining. This band corresponds to earlier findings in literature with different antibodies (PMID |
| Reference(s) | Guo Y, Cheong N, Zhang Z, De Rose R, Deng Y, Farber SA, Fernandes-Alnemri T, Alnemri ES. Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. J Biol Chem. 2004 Jun 4;279(23) |
