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Goat anti-STAP2 / BKS Antibody


Item#:
dAP-0522
Size Price Quantity
50.0 ug $310.00
Goat anti-STAP2 / BKS Antibody
Principle Name STAP2 / BKS
Official Symbol STAP2
All Name and Symbols STAP2; BKS; STAP-2; FLJ20234; breast tumor kinase substrate; brk kinase substrate; signal-transducing adaptor protein-2; signal transducing adaptor family member 2
Accession Number(s) NP_060190.2; NP_001013863.1
Peptide Sequence ELQKKLEKRRALEH, is from C Terminus
Human Gene ID(s) 55620
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 48kDa band observed in Human Heart lysates and 45kDa band observed in Human Breast Lysates (calculated MW of 49.6kDa according to NP_060190 and of NP_001013863.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Brain (Cortex) shows textured cytoplasm staining in neuronal bodies and processes. Recommended concentration, 1-2µg/ml. Data obtained from previous batch (goat).
Reference(s) Minoguchi M, Minoguchi S, Aki D, Joo A, Yamamoto T, Yumioka T, Matsuda T, Yoshimura A. STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif. J Biol Chem. 2003 Mar 28;278(13)

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