Goat anti-SART1 Antibody |
| Principle Name | SART1 |
| Official Symbol | SART1 |
| All Name and Symbols | SART1; squamous cell carcinoma antigen recognised by T cells; ARA1; Ara1; HOMS1; MGC2038; SART1259; Snu66; IgE autoantigen; SART1(259) protein; SART1(800) protein; U4/U6.U5 tri-snRNP-associated 110 kDa protein; squamous cell carcinoma antigen recognised b |
| Accession Number(s) | NP_005137.1 |
| Peptide Sequence | GSSKKHRGEKEAA, is from N Terminus |
| Human Gene ID(s) | 9092 |
| Non-Human GeneID(s) | 20227 (mouse); 29678 (rat); |
| Important Comments | This antibody is expected to recognise both the human SART1(800) and SART1(259) proteins. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 125kDa band observed in HeLa lysates (consistent with Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137). Recommended concentration |
| Reference(s) | Vertegaal AC, Ogg SC, Jaffray E, Rodriguez MS, Hay RT, Andersen JS, Mann M, Lamond AI. A proteomic study of SUMO-2 target proteins. J Biol Chem. 2004 Aug 6;279(32) |
