Goat anti-DKK1 Antibody |
| Principle Name | DKK1 |
| Official Symbol | DKK1 |
| All Name and Symbols | DKK1; SK; DKK-1; dickkopf homolog 1 (Xenopus laevis); dickkopf-1 like; dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1; dickkopf related protein-1 |
| Accession Number(s) | NP_036374.1 |
| Peptide Sequence | CDHHQASNSSRLHT, is from C Terminus |
| Human Gene ID(s) | 22943 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 38kDa band observed in Human Bone Marrow and Spleen lysates (calculated MW of 28.7kDa according to NP_036374.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fedi et al, J Bio |
| Immunohistochemistry | Paraffin embedded Human Placenta. Recommended concentration |
| Reference(s) | Fedi P, Bafico A, Nieto Soria A, Burgess WH, Miki T, Bottaro DP, Kraus MH, Aaronson SA. Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling. J Biol Chem. 1999 Jul 2;274(27) |
