Goat anti-CRKL Antibody |
| Principle Name | CRKL |
| Official Symbol | CRKL |
| All Name and Symbols | CRKL; v-crk sarcoma virus CT10 oncogene homolog (avian)-like; v-crk avian sarcoma virus CT10 oncogene homolog-like; OTTHUMP00000198539 |
| Accession Number(s) | NP_005198.1 |
| Peptide Sequence | KIFDPQNPDENE, is from C Terminus |
| Human Gene ID(s) | 1399 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 37kDa band observed in lysates of cell lines K562 (calculated MW of 33.8kDa according to NP_005198). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Recommended concentration |
| Reference(s) | Singer CF, Hudelist G, Lamm W, Mueller R, Handl C, Kubista E, Czerwenka K. Active (p)CrkL is overexpressed in human malignancies |
