Goat anti-UBE2I Antibody |
| Principle Name | UBE2I |
| Official Symbol | UBE2I |
| All Name and Symbols | UBE2I; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); C358B7.1; P18; UBC9; SUMO-1-protein ligase; ubiquitin carrier protein; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I; ubiquitin-conjugating enzyme E2I (homologous to yeast U |
| Accession Number(s) | NP_003336.1 |
| Peptide Sequence | SGIALSRLAQERK, is from N Terminus |
| Human Gene ID(s) | 7329 |
| Non-Human GeneID(s) | 22196 (mouse); 25573 (rat); |
| Important Comments | Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC, ICC |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 17kDa band observed in Human Kidney, Lung and Testis lysates and in Mouse and Rat Heart, Spleen and Testis lysates (calculated MW of 18.0kDa according to Human NP_003336.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration |
| Reference(s) | Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T, Deuel TF. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J Biol Chem. 1996 Oct 4;271(40) |
