Goat anti-LNK / SH2B3 Antibody

Item#:

dAP-0513

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-LNK / SH2B3 Antibody
Principle Name	LNK / SH2B3
Official Symbol	SH2B3
All Name and Symbols	SH2B3; LNK; SH2B adaptor protein 3; lymphocyte adaptor protein
Accession Number(s)	NP_005466.1
Peptide Sequence	NGPALQPSSPC, is from N Terminus
Human Gene ID(s)	10019
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 70kDa band observed in lysates of cell line MOLT4 (calculated MW of 63.2kDa according to NP_005466.1). In transfected HEK293 transiently expressing SH2B3 a band of approx. 65kDa is observed. This band is not observed in the non-trans
Reference(s)	Li Y, He X, Schembri-King J, Jakes S, Hayashi J. Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation. J Immunol. 2000 May 15;164(10)