Goat anti-Pirin Antibody |
| Principle Name | Pirin |
| Official Symbol | PIR |
| All Name and Symbols | PIR; pirin; pirin (iron-binding nuclear protein) |
| Accession Number(s) | NP_003653.1 |
| Peptide Sequence | GSSKKVTLSVLSREC, is from N Terminus |
| Human Gene ID(s) | 8544 |
| Non-Human GeneID(s) | |
| Important Comments | Variants (NP_003653.1; NP_001018119.1) encode the same protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 33kDa band observed in human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). In transfected HEK293 transiently expressing PIR a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK |
| Reference(s) | Wendler WM, Kremmer E, Forster R, Winnacker EL. Identification of pirin, a novel highly conserved nuclear protein. J Biol Chem. 1997 Mar 28;272(13) |
