Goat anti-RASIP1 / RAIN Antibody

Item#:

dAP-0502

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-RASIP1 / RAIN Antibody
Principle Name	RASIP1 / RAIN
Official Symbol	RASIP1
All Name and Symbols	RASIP1; Ras-interacting protein 1; RAIN; FLJ20401; Ras interacting protein 1
Accession Number(s)	NP_060275.2
Peptide Sequence	CANYRHGPPVATSP, is from C Terminus
Human Gene ID(s)	54922
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Experiments gave bands at approx 95kDa, 75kDa and 45kDa in A549 Lung Carcinoma lysate after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given th
Immunohistochemistry	Paraffin embedded Human Placenta. Recommended concentration
Reference(s)	Natalia Y. Mitin, Melissa B. Ramocki, Alfred J. Zullo, Channing J. Der, Stephen F. Konieczny, and Elizabeth J. Taparowsky Identification and Characterization of Rain, a Novel Ras-interacting Protein with a Unique Subcellular Localization J. Biol. Chem. 2004;279 22353-22361.PMID