Goat anti-Smooth muscle alpha-actin Antibody

Item#:

dAP-0501

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Smooth muscle alpha-actin Antibody
Principle Name	Smooth muscle alpha-actin
Official Symbol	ACTA2
All Name and Symbols	ACTA2; actin, alpha 2, smooth muscle, aorta; OTTHUMP00000020042; ACTSA; AAT6; ACTSA; alpha 2 actin; alpha-cardiac actin; growth-inhibiting gene 46; smooth muscle alpha-actin;
Accession Number(s)	NP_001604.1; NP_001135417.1
Peptide Sequence	EEEDSTALVC, is from N Terminus
Human Gene ID(s)	59
Non-Human GeneID(s)
Important Comments	Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human, Rat
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Approx 45kDa band observed in Human and Rat Duodenum lysates and approx. 40-45kDa in lysates oif cell line HeLa (calculated MW of 42kDa according to Human NP_001604.and Rat NP_112266.1). Recommended concentration
Reference(s)	Reddy S, Ozgur K, Lu M, Chang W, Mohan SR, Kumar CC, Ruley HE. Structure of the human smooth muscle alpha-actin gene. Analysis of a cDNA and 5' upstream region. J Biol Chem. 1990 Jan 25;265(3)