Goat anti-KLF4 / GKLF Antibody

Item#:

dAP-0489

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-KLF4 / GKLF Antibody
Principle Name	KLF4 / GKLF
Official Symbol	KLF4
All Name and Symbols	KLF4; GKLF; Kruppel-like factor 4 (gut); EZF; endothelial Kruppel-like zinc finger protein
Accession Number(s)	NP_004226.2
Peptide Sequence	AVSDALLPSFST, is from N Terminus
Human Gene ID(s)	9314
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Experiments gave strong bands at 75-90kDa and a faint 50-kDa band in Human Duodenal lysate after 0.1µg/ml antibody staining. These bands correspond to earlier findings in literature (Piccinni et al, J. Biol. Chem., Vol. 279 (10), 9103-9114)
Reference(s)	Shields JM, Christy RJ, Yang VW. Identification and characterization of a gene encoding a gut-enriched Kruppel-like factor expressed during growth arrest. J Biol Chem. 1996 Aug 16;271(33)