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Goat anti-WHIP / WRNIP1 Antibody


Item#:
dAP-0488
Size Price Quantity
50.0 ug $310.00
Goat anti-WHIP / WRNIP1 Antibody
Principle Name WHIP / WRNIP1
Official Symbol WRNIP1
All Name and Symbols WHIP; Werner helicase interacting protein; FLJ22526; putative helicase RUVBL; Werner helicase interacting protein 1; RP11-420G6.2; bA420G6.2;
Accession Number(s) NP_064520.2; NP_569079.1
Peptide Sequence EELRGVDFFKQRRC, is from C Terminus
Human Gene ID(s) 56897
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise isoform 1 (NP_064520.2) and isoform 2 (NP_569079.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 90kDa band observed in lysates of cell line Jurkat (calculated MW of 72.1kDa according to NP_064520.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bish RA, Myers MP., J Biol
Immunohistochemistry In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT. Recommended concentration, 4-6µg/ml.
Reference(s) Kawabe Yi, Branzei D, Hayashi T, Suzuki H, Masuko T, Onoda F, Heo SJ, Ikeda H, Shimamoto A, Furuichi Y, Seki M, Enomoto T. A novel protein interacts with the Werner's syndrome gene product physically and functionally. J Biol Chem. 2001 Jun 8;276(23)

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