Goat anti-Pericentrin 1 / NUP85 Antibody

Item#:

dAP-0486

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Pericentrin 1 / NUP85 Antibody
Principle Name	Pericentrin 1 / NUP85
Official Symbol	NUP85
All Name and Symbols	NUP85; pericentrin 1; PCNT1; PCN; PCNT; frount; nucleoporin Nup75; nucleoporin 85kDa; FLJ12549; Nup75; nucleoporin 85
Accession Number(s)	NP_079120.1
Peptide Sequence	ARAIIREGSLEGS, is from C Terminus
Human Gene ID(s)	79902
Non-Human GeneID(s)	18541 (mouse);
Important Comments	Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120). Recommended concentration
Reference(s)	Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M. Pericentrin, a highly conserved centrosome protein involved in microtubule organization. Cell. 1994 Feb 25;76(4)