Goat anti-Pescadillo Antibody

Item#:

dAP-0485

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Pescadillo Antibody
Principle Name	Pescadillo
Official Symbol	PES1
All Name and Symbols	PES1; pescadillo homolog 1, containing BRCT domain (zebrafish); PES; pescadillo (zebrafish) homolog 1, containing BRCT domain
Accession Number(s)	NP_055118.1
Peptide Sequence	GGLEKKKYERGSA, is from N Terminus
Human Gene ID(s)	23481
Non-Human GeneID(s)	64934 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 70kDa band observed in HeLa lysate (calculated MW of 68kDa according to NP_055118). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Testis shows nucleolar staining in spermatocytes and spermatids. Recommended concentration, 2.5-3.8µg/ml.
Reference(s)	Allende ML, Amsterdam A, Becker T, Kawakami K, Gaiano N, Hopkins N. Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. Genes Dev. 1996 Dec 15;10(24)