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Goat anti-USH1C / Harmonin Antibody
Item#:
dAP-0484
Size Price Quantity
50.0 ug $310.00
Goat anti-USH1C / Harmonin Antibody
Principle Name USH1C / Harmonin
Official Symbol USH1C
All Name and Symbols USH1C; harmonin; AIE-75; DFNB18; PDZ-45; PDZ-73; HARMONIN; NY-CO-37; NY-CO-38; PDZ-73/NY-CO-38; Usher syndrome 1C (autosomal recessive, severe); PDZ-73 protein; ush1cpst; deafness, autosomal recessive 18
Accession Number(s) NP_005700.2; NP_710142.1
Peptide Sequence DRKVAREFRHKVD, is from N Terminus
Human Gene ID(s) 10083
Non-Human GeneID(s) 72088 (mouse); 308596 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_005700.2 and NP_710142.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 75kDa band observed in lysates of cell line HEK293 (calculated MW of 62.2kDa according to NP_005700.2). Recommended concentration
Immunohistochemistry In paraffin embedded Human Kidney, shows diffuse cytopasmic staining in epithelial cells of renal tubules. Recommended concentration, 10µg/ml. (IHC data have been obtained with EB06287 from previous batch).
Reference(s) Verpy E, Leibovici M, Zwaenepoel I, Liu XZ, Gal A, Salem N, Mansour A, Blanchard S, Kobayashi I, Keats BJ, Slim R, Petit C. A defect in harmonin, a PDZ domain-containing protein expressed in the inner ear sensory hair cells, underlies Usher syndrome type 1C. Nat Genet. 2000 Sep;26(1)

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