Goat anti-KLF1 / EKLF Antibody

Item#:

dAP-0482

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-KLF1 / EKLF Antibody
Principle Name	KLF1 / EKLF
Official Symbol	KLF1
All Name and Symbols	KLF1; EKLF; Kruppel-like factor 1 (erythroid); erythroid Kruppel-like factor; INLU; monoclonal antibody A3D8
Accession Number(s)	NP_006554.1
Peptide Sequence	ATAETALPSISTLT, is from N Terminus
Human Gene ID(s)	10661
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC, ChIP
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave an approx 52kDa band in K562 cell lysate after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 38kDa accor
Immunohistochemistry	Paraffin embedded Human Bone Marrow. Recommended concentration
Reference(s)	Pandya K, Townes TM. Basic residues within the Kruppel zinc finger DNA binding domains are the critical nuclear localization determinants of EKLF/KLF-1. J Biol Chem. 2002 May 3;277(18)