Goat anti-HIP2 Antibody |
| Principle Name | HIP2 |
| Official Symbol | UBE2K |
| All Name and Symbols | UBE2K; HIP2; huntingtin interacting protein 2; LIG; HYPG; ubiquitin-protein ligase; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); DKFZp564C1216; DKFZp686J24237; E2-25K; |
| Accession Number(s) | NP_005330.1; NP_001104582.1; NP_001104583.1 |
| Peptide Sequence | SWDVETATELLLSN, is from C Terminus |
| Human Gene ID(s) | 3093 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Approx 24kDa band observed in Jurkat cell lysates (calculated MW of 22.4 kDa according to NP_005330). Recommended for use at 1-3 µg/ml. |
| Immunohistochemistry | In paraffin embedded Human Brain shows pixulate cytoplasm stainng in neurons of the Cortex. Recommended concentration, 1-2µg/ml. |
| Reference(s) | Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR. Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme. J Biol Chem. 1996 Aug 9;271(32) |
