Goat anti-RANBP1 Antibody

Item#:

dAP-0455

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-RANBP1 Antibody
Principle Name	RANBP1
Official Symbol	RANBP1
All Name and Symbols	RANBP1; RAN binding protein 1; HTF9A; MGC88701
Accession Number(s)	NP_002873.1
Peptide Sequence	LSVKEETKEDAEEKQ, is from C Terminus
Human Gene ID(s)	5902
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 26kDa band observed in lysate of cell line HeLa (calculated MW of 23.3kDa according to NP_002873.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Kidney shows pixulated cytoplasm staining in DCT. Recommended concentration, 3-5µg/ml.
Reference(s)	Bischoff FR, Krebber H, Smirnova E, Dong W, Ponstingl H. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. EMBO J. 1995 Feb 15;14(4)