Goat anti-MAML1 / Mastermind Antibody

Item#:

dAP-0454

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MAML1 / Mastermind Antibody
Principle Name	MAML1 / Mastermind
Official Symbol	MAML1
All Name and Symbols	MAML1; mastermind-like 1 (Drosophila); Mam1; Mam-1; KIAA0200; mastermind homolog; mastermind-like 1
Accession Number(s)	NP_055572.1
Peptide Sequence	VLPTCPMAEFALPR, is from N Terminus
Human Gene ID(s)	9794
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 110kDa band observed in Human Skeletal Muscle lysates (calculated MW of 108kDa according to NP_055572.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8µg/ml.
Reference(s)	Wu L, Aster JC, Blacklow SC, Lake R, Artavanis-Tsakonas S, Griffin JD. MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors. Nat Genet. 2000 Dec;26(4)