Goat anti-BIM Antibody |
| Principle Name | BIM |
| Official Symbol | BCL2L11 |
| All Name and Symbols | BCL2L11; BCL2-like 11 (apoptosis facilitator); BAM; BIM; BOD; bcl-2 interacting mediator of cell death; bcl-2 interacting protein Bim; bcl-2-like protein 11; bcl-2-related ovarian death agonist; bcl2-L-11; Bim-alpha1, Bim-alpha2, Bim-alpha3 |
| Accession Number(s) | NP_619528.1; NP_619529.1; NP_996886.1; |
| Peptide Sequence | FNAYYARRLEK, is from C Terminus |
| Human Gene ID(s) | 10018 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise at least reported isoforms Bim-alpha1 (BimABCD), Bim-alpha2 (BimACD) and Bim-alpha3 (BimAD). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 23kDa band observed in K562 lysates (predicted MW of 18.5kDa according to NP_619528.1). Recommended for use at 0.5-2µg/ml. |
| Reference(s) | Marani M, Tenev T, Hancock D, Downward J, Lemoine NR. Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis. Mol Cell Biol. 2002 Jun;22(11) |
