Goat anti-FBL2 / FBXL2 Antibody

Item#:

dAP-0449

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-FBL2 / FBXL2 Antibody
Principle Name	FBL2 / FBXL2
Official Symbol	FBXL2
All Name and Symbols	FBXL2; FBL2; F-box and leucine-rich repeat protein 2; FBL3; DKFZP564P0622; F-box protein containing leucine-rich repeats
Accession Number(s)	NP_036289.2
Peptide Sequence	VFSNNDEGLINKK, is from N Terminus
Human Gene ID(s)	25827
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Rat
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 48kDa band observed in Human Brain (Frontal Cortex) lysates and approx 50kDa band observed in Rat Brain lysates (calculated MW of 47.1kDa according to Human NP_036289.2, and 46.9kDa according to Rat XP_343496.4). Recommended concentr
Immunohistochemistry	In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in neuronal bodies. Recommended concentration, 5-10µg/ml.
Reference(s)	Ilyin GP, Rialland M, Pigeon C, Guguen-Guillouzo C. cDNA cloning and expression analysis of new members of the mammalian F-box protein family. Genomics. 2000 Jul 1;67(1)