Goat anti-TRAC-1 / RNF125 Antibody

Item#:

dAP-0447

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-TRAC-1 / RNF125 Antibody
Principle Name	TRAC-1 / RNF125
Official Symbol	RNF125
All Name and Symbols	RNF125; FLJ20456; MGC21737; ring finger protein 125; TRAC1; T-cell ring protein identified in activation screen
Accession Number(s)	NP_060301.2
Peptide Sequence	RSLLEYVNHSNTT, is from C Terminus
Human Gene ID(s)	54941
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Preliminary experiments gave no signal but low background in Human Brain and Ovary lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Reference(s)	Chu P, Pardo J, Zhao H, Li CC, Pali E, Shen MM, Qu K, Yu SX, Huang BC, Yu P, Masuda ES, Molineaux SM, Kolbinger F, Aversa G, De Vries J, Payan DG, Liao XC. Systematic identification of regulatory proteins critical for T-cell activation. J Biol. 2003;2(3)