Goat anti-ILK Antibody |
| Principle Name | ILK |
| Official Symbol | ILK |
| All Name and Symbols | ILK; integrin-linked kinase; P59; DKFZp686F1765 |
| Accession Number(s) | NP_004508.1; NP_001014794.1; NP_001014795.1 |
| Peptide Sequence | DMIVPILEKMQDK, is from C Terminus |
| Human Gene ID(s) | 3611 |
| Non-Human GeneID(s) | |
| Important Comments | All 3 varients (represented by NP_004508.1; NP_001014794.1 and NP_001014795.1) encode the same protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 52kDa band observed in Mouse Kidney lysates and in lysates of cell line NIH3T3 and 50-51kDa in HEK293, A431, HeLa and Jurkat lysates (calculated MW of 51.4kDa according to Human NP_004508.1 and Mouse NP_034692.2). Recommended concent |
| Reference(s) | Hannigan GE, Leung-Hagesteijn C, Fitz-Gibbon L, Coppolino MG, Radeva G, Filmus J, Bell JC, Dedhar S. Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase. Nature. 1996 Jan 4;379(6560) |
