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Goat anti-GCIP / MAID Antibody
Item#:
dAP-0433
Size Price Quantity
50.0 ug $310.00
Goat anti-GCIP / MAID Antibody
Principle Name GCIP / MAID
Official Symbol CCNDBP1
All Name and Symbols CCNDBP1; cyclin D-type binding-protein 1; DIP1; GCIP; HHM Protein; MAID protein; grap2 cyclin interacting protein; D-type cyclin-interacting protein 1
Accession Number(s) NP_036274.3; NP_411241.1
Peptide Sequence NRIKELTQSELEL, is from C Terminus
Human Gene ID(s) 23582
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both human isoforms of this protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 55kDa band in K562 lysate at 0.5ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the
Immunohistochemistry In paraffin embedded Human Prostate shows textured cytoplasm staining with emphasis on the luminal side of the secretory cells in the glands. Recommended concentration, 3-6µg/ml.
Reference(s) Xia C, Bao Z, Tabassam F, Ma W, Qiu M, Hua S, Liu M. GCIP, a novel human grap2 and cyclin D interacting protein, regulates E2F-mediated transcriptional activity. J Biol Chem. 2000 Jul 7;275(27)

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