Goat anti-ZIC5 Antibody |
| Principle Name | ZIC5 |
| Official Symbol | ZIC5 |
| All Name and Symbols | ZIC5; zinc finger protein of the cerebellum 5; zinc family member 5 protein; Zic family member 5 (odd-paired homolog, Drosophila) |
| Accession Number(s) | NP_149123.2 |
| Peptide Sequence | EIYGNPEVVRTIH, is from C Terminus |
| Human Gene ID(s) | 85416 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Preliminary experiments gave an approx 45kDa band in human brain lysates at 1ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for |
| Immunohistochemistry | In paraffin embedded Human Small Intestine shows nuclear staining. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Furushima K, Murata T, Matsuo I, Aizawa S. A new murine zinc finger gene, Opr. Mech Dev. 2000 Nov;98(1-2) |
