Goat anti-UBE2F/NEDD8-conjugating enzyme Antibody

Item#:

dAP-0422

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-UBE2F/NEDD8-conjugating enzyme Antibody
Principle Name	UBE2F/NEDD8-conjugating enzyme
Official Symbol	UBE2F
All Name and Symbols	UBE2F; ubiquitin-conjugating enzyme E2F (putative); MGC18120; NCE2; NEDD8 conjugating enzyme; NEDD8-conjugating enzyme
Accession Number(s)	NP_542409.1
Peptide Sequence	RNKVDDYIKRYAR, is from C Terminus
Human Gene ID(s)	140739
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise isoform 1 (NP_542409.1) only.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave no signal but low background in Human Kidney and Liver lysates at up to 1µg/ml.
Reference(s)	Podust VN, Brownell JE, Gladysheva TB, Luo RS, Wang C, Coggins MB, Pierce JW, Lightcap ES, Chau V. A Nedd8 conjugation pathway is essential for proteolytic targeting of p27Kip1 by ubiquitination. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9)