Goat anti-EPS8 Antibody |
| Principle Name | EPS8 |
| Official Symbol | EPS8 |
| All Name and Symbols | EPS8; epidermal growth factor receptor pathway substrate 8 |
| Accession Number(s) | NP_004438.3 |
| Peptide Sequence | SGVESFDEGSSH, is from C Terminus |
| Human Gene ID(s) | 2059 |
| Non-Human GeneID(s) | 13860 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? |
| Immunohistochemistry | In paraffin embedded Human Breast Carcinoma shows staining of cancer cells infiltrated in a breast lobe. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Fazioli F, Minichiello L, Matoska V, Castagnino P, Miki T, Wong WT, Di Fiore PP. Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals. EMBO J. 1993 Oct;12(10) |
