Goat anti-PAX3 Antibody |
| Principle Name | PAX3 |
| Official Symbol | PAX3 |
| All Name and Symbols | PAX3; WS1; CDHS; HUP2; paired box gene 3 (Waardenburg syndrome 1); paired domain gene 3; paired box homeotic gene 3 (Waardenburg syndrome 1); paired box 3; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box homeotic gene 3; paired domain ge |
| Accession Number(s) | NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1 |
| Peptide Sequence | TTLAGAVPRMM, is from N Terminus |
| Human Gene ID(s) | 5077 |
| Non-Human GeneID(s) | 18505 (mouse); 114502 (rat); |
| Important Comments | This antibody is expected to recognize all 8 reported isoforms (NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 45kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2kDa according to NP_852125.1 and of 44.8kDa according to NP_852126.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration |
| Reference(s) | Tsukamoto K, Nakamura Y, Niikawa N. Isolation of two isoforms of the PAX3 gene transcripts and their tissue-specific alternative expression in human adult tissues. Hum Genet. 1994 Mar;93(3) |
