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Goat anti-CPT1B (isoform 1) Antibody
Item#:
dAP-0410
Size Price Quantity
50.0 ug $310.00
Goat anti-CPT1B (isoform 1) Antibody
Principle Name CPT1B (isoform 1)
Official Symbol CPT1B
All Name and Symbols CPT1B; carnitine palmitoyltransferase I, muscle; carnitine palmitoyltransferase 1B (muscle) ; CPT1-M; M-CPT1; KIAA1670; CPTI, muscle; carnitine O-palmitoyltransferase I, mitochondrial muscle isoform; carnitine palmitoyltransferase 1A (muscle); carnitine p
Accession Number(s) NP_689452.1; NP_689451.1; NP_004368.1; NP_001138609.1; NP_001138607.1; NP_001138606.1; NP_001138608.1
Peptide Sequence DIADLFQVPKAYS, is from C Terminus
Human Gene ID(s) 1375
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise human isoforms a (NP_689452.1; NP_689451.1; NP_004368.1; NP_001138609.1; NP_001138607.1), isoform c (NP_001138606.1) and isoform d (NP_001138608.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave no signal but low background in human kidney and HepG2 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry In paraffin embedded Human Heart shows staining of myocardial fibres in longitudinal section. Recommended concentration, 3-5µg/ml.
Reference(s) Yamazaki N, Shinohara Y, Shima A, Yamanaka Y, Terada H. Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I. Biochim Biophys Acta. 1996 Jun 7;1307(2)

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