Goat anti-FYN Antibody |
| Principle Name | FYN |
| Official Symbol | FYN |
| All Name and Symbols | FYN; SLK; SYN; MGC45350; FYN oncogene related to SRC, FGR, YES; src-like kinase; c-syn protooncogene; tyrosine kinase p59fyn(T); src/yes-related novel gene; OKT3-induced calcium influx regulator; proto-oncogene tyrosine-protein kinase fyn; RP1-66H14.1; OT |
| Accession Number(s) | NP_002028.1; NP_694592.1; NP_694593.1 |
| Peptide Sequence | VQCKDKEATKLTE, is from N Terminus |
| Human Gene ID(s) | 2534 |
| Non-Human GeneID(s) | 14360 (mouse); 25150 (rat); |
| Important Comments | This antibody is expected to recognise all three reported isoforms (as represented by NP_002028.1; NP_694592.1; NP_694593.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 55kDa band observed in lysates of cell lines A431 and Jurkat (calculated MW of 54.5kDa according to NP_694593.1). Recommended for use at 0.3-1µg/ml. |
| Reference(s) | Semba K, Nishizawa M, Miyajima N, Yoshida MC, Sukegawa J, Yamanashi Y, Sasaki M, Yamamoto T, Toyoshima K. yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family. Proc Natl Acad Sci U S A 1986 Aug;83(15) |
