Goat anti-NOXO1 Antibody |
| Principle Name | NOXO1 |
| Official Symbol | NOXO1 |
| All Name and Symbols | NOXO1; P41NOX; P41NOXA; P41NOXB; P41NOXC; MGC20258; NADPH oxidase organizer 1; regulatory protein P41NOX; SH3PXD5; SNX28; Nox organizer 1 |
| Accession Number(s) | NP_653204.1; NP_751907.1; NP_751907.1 |
| Peptide Sequence | RGCVDSVPHPTTEQ, is from C Terminus |
| Human Gene ID(s) | 124056 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave no signal but low background in Hela and Human Heart lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? |
| Immunohistochemistry | In paraffin embedded Human Kidney Cortex shows cytoplasmic stain in epithelial cells of some PCT. Recommended concentration, 10-20µg/ml. |
| Reference(s) | Banfi B, Clark RA, Steger K, Krause KH. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. J Biol Chem. 2003 Feb 7;278(6) |
