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Goat anti-Syntenin 2 Antibody
Item#:
dAP-0395
Size Price Quantity
50.0 ug $310.00
Goat anti-Syntenin 2 Antibody
Principle Name Syntenin 2
Official Symbol SDCBP2
All Name and Symbols SDCBP2; ST-2; SITAC18; syndecan binding protein (syntenin) 2; syntenin-2; FLJ12256; OTTHUMP00000029961; OTTHUMP00000029964; syndecan binding protein 2
Accession Number(s) NP_056500.3; NP_536737.3
Peptide Sequence LLHHTMDHSIPDA, is from C Terminus
Human Gene ID(s) 27111
Non-Human GeneID(s) 228765 (mouse);
Important Comments This antibody is expected to recognise both human isoforms (NP_056500; NP_536737).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave no signal but low background in Human Brain and Liver lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry In paraffin embedded Human Liver shows staining of cytoplasm in hepatocytes. Recommended concentration, 4-6µg/ml.
Reference(s) Koroll M, Rathjen FG, Volkmer H. The neural cell recognition molecule neurofascin interacts with syntenin-1 but not with syntenin-2, both of which reveal self-associating activity. J Biol Chem. 2001 Apr 6;276(14)

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