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Goat anti-ASC / TMS1 Antibody
Item#:
dAP-0394
Size Price Quantity
50.0 ug $310.00
Goat anti-ASC / TMS1 Antibody
Principle Name ASC / TMS1
Official Symbol PYCARD
All Name and Symbols PYCARD; ASC; TMS1; PYD and CARD domain containing; CARD5; MGC10332; TMS; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain protein 5; target of methylation-induced silencing-1; TMS-1
Accession Number(s) NP_037390.2; NP_660183.1
Peptide Sequence RESQSYLVEDLERS, is from C Terminus
Human Gene ID(s) 29108
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx. 24kDa band observed in lysates of cell line U937 (calculated MW of 21.6kDa according to NP_037390.2). Recommended for use at 1-3µg/ml.
Immunohistochemistry In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration
Reference(s) Ohtsuka T, Ryu H, Minamishima YA, Macip S, Sagara J, Nakayama KI, Aaronson SA, Lee SW. ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway. Nat Cell Biol. 2004 Feb;6(2)

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