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Goat anti-MID2 / TRIM1 Antibody
Item#:
dAP-0390
Size Price Quantity
50.0 ug $310.00
Goat anti-MID2 / TRIM1 Antibody
Principle Name MID2 / TRIM1
Official Symbol MID2
All Name and Symbols MID2; TRIM1; midline 2; FXY2; midin 2; tripartite motif protein 1; RP6-191P20.2; FLJ37715; FLJ41813; FXY2; RNF60;
Accession Number(s) NP_036348.2; NP_438112.2
Peptide Sequence QESPYVSGMKTCH, is from C Terminus
Human Gene ID(s) 11043
Non-Human GeneID(s) 23947 (mouse);
Important Comments This antibody is expected to recognise both human isoforms of this protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10µg/ml.
Reference(s) Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B. MID2, a homologue of the Opitz syndrome gene MID1

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