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Goat anti-BAF57 / SMARCE1 Antibody
Item#:
dAP-0389
Size Price Quantity
50.0 ug $310.00
Goat anti-BAF57 / SMARCE1 Antibody
Principle Name BAF57 / SMARCE1
Official Symbol SMARCE1
All Name and Symbols SMARCE1; BAF57; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1; mammalian chromatin remodeling complex BRG1-associated factor 57; BAF57; SWI/SNF-related matrix-associated actin-dependent regulator of chro
Accession Number(s) NP_003070.3
Peptide Sequence PPTDPIPEDEKKE, is from C Terminus
Human Gene ID(s) 6605
Non-Human GeneID(s) 57376 (mouse);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 50kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6kDa according to NP_003070.3). A 48kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the
Immunohistochemistry In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration, 3-5µg/ml.
Reference(s) Wang W, Chi T, Xue Y, Zhou S, Kuo A, Crabtree GR. Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes. Proc Natl Acad Sci U S A. 1998 Jan 20;95(2)

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