Goat anti-PACSIN3 Antibody

Item#:

dAP-0387

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PACSIN3 Antibody
Principle Name	PACSIN3
Official Symbol	PACSIN3
All Name and Symbols	PACSIN3; protein kinase C and casein kinase substrate in neurons 3; SH3 domain-containing protein 6511; SDPIII; syndapin III; SH3 domain-containing protein 6511
Accession Number(s)	NP_057307.2
Peptide Sequence	APEEDAGGEALGGS, is from N Terminus
Human Gene ID(s)	29763
Non-Human GeneID(s)	80708 (mouse); 311187 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	EB05271 (0.03µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Reference(s)	Cuajungco MP, Grimm C, Oshima K, D'hoedt D, Nilius B, Mensenkamp AR, Bindels RJ, Plomann M, Heller S. PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4. J Biol Chem. 2006 Jul 7;281(27)