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Goat anti-FAPP2 / PLEKHA8 Antibody
Item#:
dAP-0383
Size Price Quantity
50.0 ug $310.00
Goat anti-FAPP2 / PLEKHA8 Antibody
Principle Name FAPP2 / PLEKHA8
Official Symbol PLEKHA8
All Name and Symbols PLEKHA8; FAPP2; phosphoinositol 4-phosphate adaptor protein-2; MGC3358; pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8
Accession Number(s) NP_116028.1
Peptide Sequence DIQTALRNPTENT, is from C Terminus
Human Gene ID(s) 84725
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Preliminary experiments gave no signal but low background in Human Kidney and Testis lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry In paraffin embedded Human Placenta shows strong staining of cytoplasm in the margins of villi. Recommended concentration, 2-4µg/ml.
Reference(s) Dowler S, Currie RA, Campbell DG, Deak M, Kular G, Downes CP, Alessi DR. Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities. Biochem J. 2000 Oct 1;351(Pt 1)

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