Goat anti-ACADM Antibody |
| Principle Name | ACADM |
| Official Symbol | ACADM |
| All Name and Symbols | ACADM; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; RP4-682C21.1; ACAD1; MCAD; MCADH; medium-chain acyl-CoA dehydrogenase; FLJ18227; FLJ93013; FLJ99884; medium-chain specific acyl-CoA dehydrogenase |
| Accession Number(s) | NP_000007.1; NP_001120800.1 |
| Peptide Sequence | RLIVAREHIDKYKN, is from C Terminus |
| Human Gene ID(s) | 34 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_000007.1; NP_001120800.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 45kDa band observed in Human Kidney and Heart lysates (calculated MW of 46.6kDa according to NP_000007.1). Recommended for use at 0.01-0.1µg/ml. |
| Immunohistochemistry | In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Matsubara Y, Kraus JP, Yang-Feng TL, Francke U, Rosenberg LE, Tanaka K. Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1. Proc Natl Acad Sci U S A. 1986 Sep;83(17) |
