Goat anti-PAX8 (N Terminus) Antibody

Item#:

dAP-0373

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PAX8 (N Terminus) Antibody
Principle Name	PAX8 (N Terminus)
Official Symbol	PAX8
All Name and Symbols	PAX8; paired box gene 8; paired domain gene 8; paired box 8
Accession Number(s)	NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1
Peptide Sequence	PHNSIRSGHGGLN, is from N Terminus
Human Gene ID(s)	7849
Non-Human GeneID(s)	18510 (mouse); 81819 (rat);
Important Comments	This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 35kDa band observed in Human Kidney lysates. An additional 48kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9kDa according to Human NP_039247.1 PAX8D and 48.2kDa according to Human NP_003457.1 PAXA a
Reference(s)	Pasca di Magliano M, Di Lauro R, Zannini M. Pax8 has a key role in thyroid cell differentiation. Proc Natl Acad Sci U S A. 2000 Nov 21;97(24)