Goat anti-CHFR Antibody |
| Principle Name | CHFR |
| Official Symbol | CHFR |
| All Name and Symbols | CHFR; checkpoint with forkhead and ring finger domains; FLJ10796; FLJ33629; RNF116; RNF196; E3 ubiquitin-protein ligase CHFR; RING finger protein 196 |
| Accession Number(s) | NP_001154816.1; NP_001154817.1; NP_001154818.1; NP_060693.2; NP_001154819.1 |
| Peptide Sequence | KFNHICEQTRFKN, is from C Terminus |
| Human Gene ID(s) | 55743 |
| Non-Human GeneID(s) | 231600 (mouse); 288734 (rat); |
| Important Comments | This antibody is expected to recognise all reported isoforms. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature |
| Immunohistochemistry | In paraffin embedded Human Brain (Cerebellum) shows stained Purkinje cells. Recommended concentration |
| Reference(s) | Scolnick DM, Halazonetis TD. Chfr defines a mitotic stress checkpoint that delays entry into metaphase. Nature. 2000 Jul 27;406(6794) |
