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Goat anti-AIRE (isoforms 1 and 2) Antibody
Item#:
dAP-0369
Size Price Quantity
50.0 ug $310.00
Goat anti-AIRE (isoforms 1 and 2) Antibody
Principle Name AIRE (isoforms 1 and 2)
Official Symbol AIRE
All Name and Symbols AIRE; autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy); APS1; APSI; PGA1; APECED; AIRE protein; autoimmune regulator (APECED protein); autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrop
Accession Number(s) NP_000374.1, NP_000649.1
Peptide Sequence QSMARPAAPFPS, is from C Terminus
Human Gene ID(s) 326
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 60kDa band observed in Human Lymph Node and Human Spleen lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration
Reference(s) Kumar PG, Laloraya M, Wang CY, Ruan QG, Davoodi-Semiromi A, Kao KJ, She JX. The autoimmune regulator (AIRE) is a DNA-binding protein. J Biol Chem. 2001 Nov 2;276(44)

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