Goat anti-BIRC6 / Apollon Antibody

Item#:

dAP-0367

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-BIRC6 / Apollon Antibody
Principle Name	BIRC6 / Apollon
Official Symbol	BIRC6
All Name and Symbols	BIRC6; baculoviral IAP repeat-containing 6 (apollon); KIAA1289; baculoviral IAP repeat-containing 6; BRUCE; APOLLON; FLJ13726; FLJ13786; KIAA1289; ubiquitin-conjugating BIR-domain enzyme apollon
Accession Number(s)	NP_057336.3
Peptide Sequence	PSSSKELPSDFQL, is from C Terminus
Human Gene ID(s)	57448
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments gave no signal but low background in Human Liver and Kidney lysates at up to 1µg/ml. However this used our routine western blotting protocol which we would not expect to reliably detect proteins as large as the predi
Immunohistochemistry	Paraffin embedded Human Lung. Recommended concentration
Reference(s)	Chen Z, Naito M, Hori S, Mashima T, Yamori T, Tsuruo T. A human IAP-family gene, apollon, expressed in human brain cancer cells. Biochem Biophys Res Commun. 1999 Nov 2;264(3)