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Goat anti-ABCB9 / TAPL Antibody
Item#:
dAP-0364
Size Price Quantity
50.0 ug $310.00
Goat anti-ABCB9 / TAPL Antibody
Principle Name ABCB9 / TAPL
Official Symbol ABCB9
All Name and Symbols ABCB9; ATP-binding cassette, sub-family B (MDR/TAP), member 9; TAPL; KIAA1520; EST122234
Accession Number(s) NP_062570.1; NP_062571.1; NP_982269.1
Peptide Sequence GHNEPVANGSHKA, is from C Terminus
Human Gene ID(s) 23457
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both human isoforms of this protein (as represented by NP_062570.1, NP_062571.1and NP_982269.1). Variants (NP_062571.1; NP_982269.1) encode the same isoform.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Preliminary experiments gave no signal but low background in human brain and liver lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry In paraffin embedded Human Testis shows vesiculate cytoplasm staining of primary spermatocytes. Recommended concentration, 3-5µg/ml.
Reference(s) Zhang F, Zhang W, Liu L, Fisher CL, Hui D, Childs S, Dorovini-Zis K, Ling V. Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes. J Biol Chem. 2000 Jul 28;275(30)

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