Goat anti-EndoNEDD4 / NEDL2 Antibody

Item#:

dAP-0361

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-EndoNEDD4 / NEDL2 Antibody
Principle Name	EndoNEDD4 / NEDL2
Official Symbol	HECW2
All Name and Symbols	HECW2; HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2; NEDD4-related E3 ubiquitin ligase NEDL2; KIAA1301 protein; EndoNEDD4; DKFZp686M17164; NEDL2
Accession Number(s)	NP_065811.1
Peptide Sequence	ASSAREHLLFVRR, is from N Terminus
Human Gene ID(s)	57520
Non-Human GeneID(s)	227106 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave no signal but low background in Human Lung and Kidney lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Reference(s)	Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 2000 Feb 28;7(1)