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Goat anti-Flotillin 1 Antibody
Item#:
dAP-0360
Size Price Quantity
50.0 ug $310.00
Goat anti-Flotillin 1 Antibody
Principle Name Flotillin 1
Official Symbol FLOT1
All Name and Symbols FLOT1; flotillin 1; reggie-2; integral membrane component of caveolae; DADB-118P11.2
Accession Number(s) NP_005794.1; NP_001305804.1
Peptide Sequence SISQVNHKPLRTA, is from C Terminus
Human Gene ID(s) 10211
Non-Human GeneID(s) 14251 (mouse);
Important Comments This antibody is expected to recognize both reported isoforms (NP_005794.1; NP_001305804.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 48kDa band observed in lysates of cell line H460 (calculated MW of 47.4kDa according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48kDa is observed. This band is not observed in the non-trans
Immunohistochemistry In paraffin embedded Human Kidney shows membrane staning in DCT. Recommended concentration, 3-5µg/ml.
Reference(s) Bickel PE, Scherer PE, Schnitzer JE, Oh P, Lisanti MP, Lodish HF. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem. 1997 May 23;272(21)

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