Goat anti-HEC1 Antibody |
| Principle Name | HEC1 |
| Official Symbol | NDC80 |
| All Name and Symbols | NDC80; HEC1; HEC; highly expressed in cancer, rich in leucine heptad repeats; HEC-1; KNTC2; NDC80 homolog, kinetochore complex component (S. cerevisiae); TID3; hsNDC80; kinetochore associated 2 |
| Accession Number(s) | NP_006092.1 |
| Peptide Sequence | YEKKATLIKSSEE, is from C Terminus |
| Human Gene ID(s) | 10403 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 75-80kDa band observed in Hela lysate (predicted MW of 74kDa according to NP_006092). Recommended for use at 0.5-2µg/ml. |
| Immunohistochemistry | Paraffin embedded Human Liver and Adrenal Gland. Recommended concentration |
| Reference(s) | Chen Y, Riley DJ, Chen PL, Lee WH. HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis. Mol Cell Biol. 1997 Oct;17(10) |
