Goat anti-ARPC2 Antibody |
| Principle Name | ARPC2 |
| Official Symbol | ARPC2 |
| All Name and Symbols | ARPC2; actin related protein 2/3 complex, subunit 2, 34kDa; ARC34; PRO2446; p34-Arc; PNAS-139; ARP2/3 protein compex subunit 34; actin related protein 2/3 complex subunit 2 |
| Accession Number(s) | NP_005722.1; NP_690601.1 |
| Peptide Sequence | EMKTITGKTFSSR, is from C Terminus |
| Human Gene ID(s) | 10109 |
| Non-Human GeneID(s) | 76709 (mouse); |
| Important Comments | The reported variants NP_005722.1 and NP_690601.1 represent identical protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 33-34kDa band observed in Hela and Human Skeletal Muscle lysates (predicted MW of 34kDa according to NP_005722). Recommended for use at 0.1-0.5µg/ml. |
| Immunohistochemistry | In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol. 1997 Jul 28;138(2) |
