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Goat anti-Dynactin Antibody
Item#:
dAP-0344
Size Price Quantity
50.0 ug $310.00
Goat anti-Dynactin Antibody
Principle Name Dynactin
Official Symbol DCTN1
All Name and Symbols DCTN1; dynactin 1 (p150, glued homolog, Drosophila); P135; DP-150; DAP-150; p150-glued; p150, Glued (Drosophila) homolog; 150 kDa dynein-associated polypeptide; dynactin 1 (p150, Glued (Drosophila) homolog); HMN7B; OTTHUMP00000202491; dynactin 1; p150, Gl
Accession Number(s) NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1
Peptide Sequence QEQLHQLHSRLIS, is from C Terminus
Human Gene ID(s) 1639
Non-Human GeneID(s) 13191 (mouse);
Important Comments This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC, ICC
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 150kDa band observed in Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2µg/ml. Primary incubated for 1 hour. A customer found positive results in WB on Mouse embryonic fibroblasts
Immunohistochemistry In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration
Reference(s) Holzbaur EL, Tokito MK. Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization. Genomics. 1996 Feb 1;31(3)

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