Goat anti-ELF1 Antibody |
| Principle Name | ELF1 |
| Official Symbol | ELF1 |
| All Name and Symbols | ELF1; E74-like factor 1 (ets domain transcription factor); OTTHUMP00000018310; ets-related transcription factor Elf-1 |
| Accession Number(s) | NP_758961.1; NP_001138825.1 |
| Peptide Sequence | AMKQNELLEPNSF, is from C Terminus |
| Human Gene ID(s) | 1997 |
| Non-Human GeneID(s) | 13709 (mouse); |
| Important Comments | This antibody is expected to recognise both reported isoforms. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 65kDa band observed in Human Lymph Node and Tonsil and in Mouse Thymus lysates (calculated MW of 64.9kDa according to Human NP_001138825.1 and 62.3kDa according to Mouse NP_001273341.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Thymus. Recommended concentration |
| Reference(s) | Wang CY, Petryniak B, Thompson CB, Kaelin WG, Leiden JM. Regulation of the Ets-related transcription factor Elf-1 by binding to the
retinoblastoma protein. Science. 1993 May 28;260(5112) |
