Goat anti-HIP-55 / SH3P7 Antibody

Item#:

dAP-0333

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-HIP-55 / SH3P7 Antibody
Principle Name	HIP-55 / SH3P7
Official Symbol	DBNL
All Name and Symbols	DBNL; HIP-55; SH3P7; src homology 3 domain-containing protein HIP-55; HIP-55 protein; drebrin F; drebrin-like; ABP1
Accession Number(s)	NP_054782.2; NP_001014436.1; NP_001116428.1
Peptide Sequence	AANLSRNGPALQE, is from N Terminus
Human Gene ID(s)	28988
Non-Human GeneID(s)	13169 (mouse); 83527 (rat);
Important Comments	This antibody is expected to recognise all reported isoforms (NP_054782.2; NP_001014436.1; NP_001116428.1)
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 55kDa band observed in Jurkat and 3T3 lysates (calculated MW of 49.0kDa according to Human NP_001116428.1 and 48.7kDa according to Mouse NP_001139780.1). Recommended for use at 1-3µg/ml.
Reference(s)	Ensenat D, Yao Z, Wang XS, Kori R, Zhou G, Lee SC, Tan TH. A novel src homology 3 domain-containing adaptor protein, HIP-55, that interacts with hematopoietic progenitor kinase 1. J Biol Chem. 1999 Nov 26;274(48)